centigrade
and Alprazolam was having 225 degrees
centigrade. By thin layer chromatography
method also both the substance can be easily
distinguished. The method used
mandatory to follow alternative
methods like Chromatography and Spectroscopy. Gas chromatography
(GC) and High performance Liquid Chromatography (HPLC) offer high
sensitivity and specificity for confirmation ... most most sensitive and specific methods for confirmation of
drug presence in a sample. The Spectroscopic methods are FT-IR with
standard library/control
mandatory to follow alternative
methods like Chromatography and Spectroscopy. Gas chromatography
(GC) and High performance Liquid Chromatography (HPLC) offer high
sensitivity and specificity for confirmation ... most most sensitive and specific methods for confirmation of
drug presence in a sample. The Spectroscopic methods are FT-IR with
standard library/control
patent IN '646 has the following 8 claims:-
"1. A method for making a composition comprising Pertuzumab and
one or more variants wherein ... determined
by hydrophobic interaction chromatography (HIC) of intact antibody.
3. The method as claimed in claim 1, wherein the amount of LMWS of
Pertuzumab
Exhibits were analyzed by Colour Test, TLC and
Gas Chromatography - Mass Spectroscopy (GC-MS)
methods. Based upon the above methods the results are
given below
Test Test Method Unit Results
No. obtained
1. Moisture AoAC Official Methods g/100g 16.07
(2012) 950.46
2. Ash AoAC Official Methods g/100g ... Threonine J. Chromatography g/100g 2.75
204:143-148
3. Serine J. Chromatography g/100g 3.46
204:143-148
4. Glutamic J. Chromatography g/100g
Gases and Propane/Propene mixtures by Gas
Chromatography is given below:
"1.Scope
1.1 This test method covers the quantitative determination of individual
hydrocarbons ... Summary of Test Method
4.1 An LPG sample is analyzed via either liquid or gas sampling valves by
gas chromatography and compared to corresponding components
Gases and Propane/Propene mixtures by Gas
Chromatography is given below:
"1.Scope
1.1 This test method covers the quantitative determination of individual
hydrocarbons ... Summary of Test Method
4.1 An LPG sample is analyzed via either liquid or gas sampling valves by
gas chromatography and compared to corresponding components
Gases and Propane/Propene mixtures by Gas
Chromatography is given below:
"1.Scope
1.1 This test method covers the quantitative determination of individual
hydrocarbons ... Summary of Test Method
4.1 An LPG sample is analyzed via either liquid or gas sampling valves by
gas chromatography and compared to corresponding components
Gases and Propane/Propene mixtures by Gas
Chromatography is given below:
"1.Scope
1.1 This test method covers the quantitative determination of individual
hydrocarbons ... Summary of Test Method
4.1 An LPG sample is analyzed via either liquid or gas sampling valves by
gas chromatography and compared to corresponding components
