showed partial amplification with
Investigator ID Plex Plux R PCR Amplification Kit.
4. DNA isolated from Exhibit1
(lower/pyjama of child victim) and Exhibit ... showed
amplification of YSTRs with Powerplex 23 R PCR
Amplification Kit.
5. DNA isolated from Exhibit2C (vaginal
swab of child victim) and Exhibit
2013/DNA3062) & '1' (Blood sample of
accused) (vide FSL 2014/DNA8218) were subjected to DNA isolation.
DNA was isolated from ... 2012/DNA8505, '1' (vide FSL2013/DNA3062 & '1' (vide
FSL2014/DNA8218). DNA profiles were generated from
DNA isolation. DNA was isolated from the source of
exhibits '1d', '1e', '1f' & '2' and DNA ... profiles were generated by using
Amp Fl Identifiler plus PCR Amplification Kit. However, DNA could
not be isolated from the source of exhibits
exhibits. The isolated DNA was checked for quality and
quantity by 0.8% agarose gel electrophoresis and ..(sic) bromide staining.
The DNA was subjected to Multiplex ... Amelogenin using Powerplex 21 PCR
Amplification Kit. The amplified products along with controls were run on
automated DNA Sequencer (ABI 3130, Applied Biosystems
Blood could not be detected on exhibit '1c'.
DNA EXAMINATION
Exhibits '1ghj' (cervical mucus swab, vaginal smear on microslides and
vaginal ... were subjected to DNA isolation. DNA was isolated from
exhibits '1ghj'. DNA was amplifies using Identifiler plus STR
amplification kit. Gene Mapper
Blood in gauze of accused) were subjected to DNA isolation.
DNA was isolated from the source of exhibits '1A' (Cervical swab ... gauze of
accused). Female DNA profile was generated from the source of exhibit
'1C' (Blood Sample). Male DNA profile was generated from the
source
were subjected to DNA examination.
Identifiler plus STR amplification kit was used for PCR amplification
and Gene Mapper IDx Software was used to DNA profiling
underwear (of victim) were subjected to DNA isolation. DNA was
isolated from Exhibit '3' i.e. blood sample (of accused Tarun Kumar ... careful perusal and analysis of the DNA Finger Printing
evidence on record, it clearly shows that the DNA (STR analysis)
profiling performed on the source
Forensic Evidence:
18. PW13 A.K. Shrivastava, Deputy Director (Biology), DNA
Fingerprinting Unit, FSL, Delhi testified that on 18.12.2012 two
parcels were received ... kit and three pads in normal saline of the
prosecutrix which was marked as Ex.2a and Ex.2b respectively. On
DNA profiling
2023 20:35:48 :::CIS
7
Report no. 1462 RFSL DNA-DNA purified from
Exhibit- 1(blood sample on FTA card, Shali Ram) and
Exhibit ... Amplification Kit.
.
Opinion:
On the basis of the analysis performed on the above
mentioned exhibits, it is concluded that:-
(i) A human male DNA profile
