Section 11(10)(d) in The Central Silk Board Silkworm Seed Regulations, 2010
(d)twenty moths shall be taken in a crushing jar and 80 ml of 0.6% K2CO3 (90 ml for dry moths) solution be added and crushed for two minutes at 10000 rpm and the jars shall be left for two minutes to settle before filtering it into centrifuge tubes and the filtrate shall be centrifuged at 3000 rpm for three minutes;