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Union of India - Section

Section 11 in The Central Silk Board Silkworm Seed Regulations, 2010

11. Quality standards for production of mulberry silkworm seeds.

(1)The seed producer shall clean, wash and disinfect the grainage rooms, premises and appliances before initiating the grainage operations.
(2)The seed producer shall procure and maintain multivoltine and bivoltine parent seed cocoons at the ratio of 2-1 for the preparation of cross breed layings.
(3)The seed producer shall preserve the seed cocoons or pupae in a single layer providing temperature of 25 ± 1°C, relative humidity of 75 ± 5% and 10 hours light and 14 hours darkness.
(4)The male and female moths shall be separated to prevent inbreeding and to facilitate crossing with the desired females and males for preparation of cross breed layings.
(5)When moths are coupled, they shall be allowed for pairing for a minimum of three hours but when male moths are used for second coupling they shall be rested for at least three hours before they are coupled and the male moths coupled for the second time shall be allowed to pair for at least four hours.
(6)The male moths shall not be used for more than two mating or pairing.
(7)Male moths shall be preserved at 7 to 9°C to retain their vigor and vitality.
(8)The moths shall be handled gently without injuring them.
(9)The female moths shall be allowed to lay eggs for twenty four hours on labeled egg sheets for oviposition at 25°C ± 1°C temperature and 75 ± 5% relative humidity in semi dark condition.
(10)The seed producer shall observe the following method of mother moth examination, to detect pebrine spores.-
(a)mother moth samples shall be collected after the moths have laid the eggs and the number of mother moths shall be sampled and examined as below.-
(i) First day - All the moths (100 %)
(ii) Second day - 20 % of the moths
(iii) Third day - 20 % of moths
(iv) Fourth day - All the moths (100 %)
(b)while collecting moth samples care shall be taken not to exclude moths with morphological deformities or moths which laid poor or deformed eggs;
(c)fresh moths stored after drying at 70°C for six hours shall be used for the examination;
(d)twenty moths shall be taken in a crushing jar and 80 ml of 0.6% K2CO3 (90 ml for dry moths) solution be added and crushed for two minutes at 10000 rpm and the jars shall be left for two minutes to settle before filtering it into centrifuge tubes and the filtrate shall be centrifuged at 3000 rpm for three minutes;
(e)the supernatant shall be rejected and the sediment be dissolved in 2-3 drops of K2CO3 (0.6%) solution and mixed well;
(f)a smear from the dissolved solution shall be placed on a glass slide using a glass rod and examined under a microscope with a magnification of 600 X to detect the pebrine spores and five fields in each smear shall be examined.
(11)If there is any incidence of pebrine disease, the seed production shall be discontinued and the eggs, if any, prepared already, shall be destroyed by burning and all prophylactic measures shall be taken to prevent contamination.
(12)The incidence of pebrine shall be informed immediately, in writing, to the jurisdictional Seed Officer with necessary details, the source of procurement, lot number, date of purchase, date of examination, stage of examination and the details of test results.
(13)After the examination all the egg sheets shall be physically verified and the deformed eggs and layings having less than two hundred and fifty eggs shall be removed.
(14)The disease free eggs shall be surface disinfected by dipping the eggs in two per cent formalin for fifteen minutes.
(15)In the case of bivoltine hybrid egg production, cocoons of the component races shall be procured and maintained in equal numbers.
(16)The seed producer shall separate male and female pupae.
(17)The separated male and female pupae shall be labeled and preserved separately in different rooms.
(18)For loose egg preparation, starch coated sheets shall be used for egg laying and the eggs shall be separated and washed thoroughly.
(19)Bivoltine eggs for immediate brushing shall be treated in hydrochloric acid with specific gravity of 1.075 for 5 minutes at 46°C or specific gravity of 1.1 for 90 minutes at 25° to prevent diapause.
(20)The acid treated eggs shall be washed thoroughly in running water to remove the acid traces completely.
(21)The washed eggs shall be dried and winnowed to remove light eggs and packed on the same day in loose egg boxes containing about 25000 eggs per box or 50 Disease free layings.
(22)The hibernated bivoltine hybrid eggs shall be cold stored for breaking diapauses.
(23)The eggs after release shall be incubated and distributed.
(24)The seed producer shall ensure that,
(a)the eggs are disease free and certified;
(b)the average number of eggs per Disease free laying are not less than four hundred and fifty;
(c)Disease free layings having less than two hundred and fifty eggs are not retained;
(d)Disease free layings having deformed eggs or egg clumps are removed;
(e)The hatching of incubated eggs are not less than ninety per cent.
(25)The seed sold by the seed producer shall be authenticated with stamping including name of the grainage, lot number, variety or kind of silkworm seed, quantity, date of laying and probable date of hatching.
(26)The seed producer shall keep the record of the test results and certify the seed produced by him to be disease free if found fit after the examination and distribute only the disease free certified silkworm seed.
(27)The seed producer shall reject the lot if he fails to recover a minimum of twenty per cent Disease free layings from the parent seed cocoons procured.