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[Cites 0, Cited by 0] [Section 11] [Entire Act]

Union of India - Subsection

Section 11(10) in The Central Silk Board Silkworm Seed Regulations, 2010

(10)The seed producer shall observe the following method of mother moth examination, to detect pebrine spores.-
(a)mother moth samples shall be collected after the moths have laid the eggs and the number of mother moths shall be sampled and examined as below.-
(i) First day - All the moths (100 %)
(ii) Second day - 20 % of the moths
(iii) Third day - 20 % of moths
(iv) Fourth day - All the moths (100 %)
(b)while collecting moth samples care shall be taken not to exclude moths with morphological deformities or moths which laid poor or deformed eggs;
(c)fresh moths stored after drying at 70°C for six hours shall be used for the examination;
(d)twenty moths shall be taken in a crushing jar and 80 ml of 0.6% K2CO3 (90 ml for dry moths) solution be added and crushed for two minutes at 10000 rpm and the jars shall be left for two minutes to settle before filtering it into centrifuge tubes and the filtrate shall be centrifuged at 3000 rpm for three minutes;
(e)the supernatant shall be rejected and the sediment be dissolved in 2-3 drops of K2CO3 (0.6%) solution and mixed well;
(f)a smear from the dissolved solution shall be placed on a glass slide using a glass rod and examined under a microscope with a magnification of 600 X to detect the pebrine spores and five fields in each smear shall be examined.