Section 12 in The Central Silk Board Silkworm Seed Regulations, 2010
12. Quality Standards for production of Tasar silkworm seed.
(1)The grainage rooms, earthen cups and other appliances shall be thoroughly cleaned, washed and disinfected.(2)The seed cocoons shall be tied in the form of garlands consisting of one hundred cocoons in twenty bunches of five cocoons each and preserved in a hanging position.(3)During summer, the temperature and humidity inside the grainage shall be maintained below 35°C and at 40-60%, respectively.(4)Seed cocoons shall be periodically screened and sorted.(5)The moths shall be allowed to mate after two to three hours of emergence in dark for four to six hours and allowed to lay eggs in earthen cups or in nylon bags.(6)Dead and old moths shall be removed every day.(7)The nylon bags in which moths are allowed to lay eggs shall be hung in bunches of two in the oviposition rooms at a temperature of 30°C and relative humidity of eighty to ninety percent.(8)The eggs shall be collected immediately after seventy two hours.(9)Mother moths shall be subjected to microscopic examination individually to detect pebrine incidence and pebrine infected moths and layings shall be destroyed.(10)Eggs shall be washed and disinfected by dipping in 0.5% NaOH solution for 40 seconds and washed thoroughly in water and washed eggs shall be dried in shade under fan and after drying shall be packed in muslin cloth bags in units of two hundred grams and labeled.(11)During transportation, the eggs shall not be exposed to temperature above 30°C and relative humidity below seventy percent.(12)The eggs prepared during the first fortnight of June every year shall be preserved at 8±2°C for eight to ten days, if necessary.(13)The seed producer shall ensure that-(a)the eggs are certified as disease free(b)the weight of eggs per Disease free laying is not less than two grams(c)the hatching of incubated eggs are not less than eighty percent.
