Section 16 in The Central Silk Board Silkworm Seed Regulations, 2010
16. Conditions to be complied with by the registered chawki silkworm rearer.
- The registered chawki silkworm rearer shall.-(i)procure only the certified hybrid seed from a silkworm seed producer that meet the quality standards as specified in these regulations;.(ii)rear and transact only the variety or kind of silkworm as indicated in the certificate of registration;(iii)not change the venue of the chawki rearing centre without the written permission of the Registration Committee;(iv)rear the chawki worms adopting the following method and procedure, namely.-(a)the number of larvae per 100 Disease free layings shall be not less than thirty eight thousand in the case of cross breed and not less than forty four thousand in the case of bivoltine hybrids;(b)the larvae shall be of uniform size and should be free from diseases;(c)maintain a mulberry garden;(d)clean, wash and disinfect the rearing house, rearing appliances and the premises before starting each rearing;(e)collect the silkworm eggs, transport during cool hours and shall protect them from direct sunlight and heat shock during transportation;(f)incubate the eggs at 25°C and eighty percent relative humidity;(g)use tender mulberry leaves for rearing the chawki silkworm larvae;(h)ensure hygiene in the chawki rearing house;(i)provide spacing according to the stage of the larvae;(j)ensure that the larvae, settle for moult uniformly and feeding is resumed in time and reject the larvae which do not settle for moult and report if such irregularity in growth and performance is more than ten percent, to the nearest Seed Officer.(k)take suitable prophylactic measures during moulting and resumption of feeding after the moult.(l)maintain temperature of 28 ± 1°C and relative humidity of 85 ± 5% in the rearing house during chawki silkworm rearing.(m)transport the chawki worms during cool hours of the day;(v)conduct testing of chawki worms as per the following procedure, namely.-(a)weak larvae shall be picked during first and second moult and homogenised with 0.6% K2CO3 solution using a mortar and pestle;(b)the homogenate shall be allowed to settle for three minutes in a beaker and filtered;(c)the sediment shall be dissolved in a few drops of 0.6 per cent K2CO3 solution and the smears shall be examined under a microscope.(vi)not refrigerate the eggs or chawki worms;(vii)maintain up to date record of hybrid seed procurement, larvae examination details, disease incidence and disposal of chawki worms and submit quarterly reports in Form-2.(viii)give free access to the records and products to the Seed Officer or Seed Analyst or any other officer so appointed or authorised by the Committee, during his visit and provide such assistance as may be required;(ix)if there is any incidence of disease, he shall discontinue the rearing and reject and destroy the entire batch of larvae and shall take all prophylactic measures to prevent contamination;(x)inform immediately such incidence in writing to the Seed Officer and seed producer with the necessary details such as the source of seed, lot number, date of purchase, date of examination, stage of examination and the details of test results;(xi)certify the chawki worms as 'tested ok' if found disease free and authenticate with stamping including his name and date of transaction;Chapter - 8
